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KMID : 1123920120260030344
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 3 p.344 ~ p.350
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Identification of the Constituents for Nrf2 Activation and NF- Suppression in Dangguisoo-san
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Kim Kyun-Ha
Jeong Ja-Haeng
Jeong Han-Sol
Ha Ki-Tae
Joo Myung-Soo
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Abstract
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Previously, we showed that Dangguisoo-san (DGSS), an herbal formula that has been traditionally used for the treatment of blood stagnation, is also applicable for inflammatory lung diseases. Activation of Nrf2, an anti-inflammatory transcription factor, and suppression of NF-κB, a pro-inflammatory transcription factor, were suggested as an underlying mechanism. However, the constituents responsible for these activities remain unidentified. To this end, we prepared the water extracts of the 9 constituents of DGSS and tested for their effect on Nrf2 by using an Nrf2-Luciferase reporter cell line and western blot analysis. Results show that Carthamus tinctorius L.(CT), one of the 9 constituents of DGSS, strongly activated Nrf2. Similarly, when measured the effect of the 9 constituents on NF-κB by using an NF-κB-Luciferase reporter cell line and western blotting for nuclear p65, indicative of activated NF-κB, most constituents were capable of suppressing NF-κB in various degrees. However, CT and Cyperus rotundus L. (CR) strongly suppressed NF-κB activity elicited by LPS. Of note, CT activated Nrf2 and suppressed NF-κB strongly as well. Our results contributes to corroborating the anti-inflammatory effects of DGSS by identifying CT and CR as two major herbs responsible for activating Nrf2 and suppressing NF-κB. These results suggest that CT and CR represent some of the effects of DGSS in the regulation of inflammation.
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KEYWORD
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anti-inflammation, Nrf2, NF-κB, carthamus tinctorius, cyperus rotundus lipopolysaccharide
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